INFLUENCE OF EMP IRRADIATION ON LDH, AST, CHE, K+ AND Na+ EN SUPERNATANT OF CULTURED RAT PI- TUITARY CELLS
- VernacularTitle:电磁脉冲辐照对培养大鼠垂体细胞上清中LDH、AST、CHE、K~+和Na~+的影响
- Author:
Xiaozhe CAO
;
Meilan ZHAO
;
Dewen WANG
- Publication Type:Journal Article
- Keywords:
radiation;
pituitary gland;
lactate dehydrogenase;
cholinesterases
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
By observing the changes in LDH、AST、CHE、K、Na+ contents in supernatant of cultured rat pituitary cells after electromagnetic pulse (EMP) irradiation the injury mechanism to pituitary cells was explored. Pituitary cells of Wistar rat was cultured in 6-hole plates. The cells in five plates were irradiated with high field strength EMP ( electromagnetic pulse) for 5 times within 2 minutes. The electromagnetic pulse simulator provided a high electric field intensity of 60 KV/m, with rise time 20-nsec and pulse wide 30/?S. Contents of LDH, AST, CHE, K+ and Na+ in the supernatant were measured using reagent boxes (Beijing Zhongsheng High-tech Bioengineering Company) at Oh, 1h, 6h, 12h and 24h following irradiation, respeetively. All data were analyzed by statistical software Spss 8. 0. The results showed that LDH and CHE contents in the supernatant increased significantly at Oh after irradiation with EMP (P 0. 05). Our conclusion is EMP could injure membranes of pituitary cells.
