Expression change of nitric oxide synthase in major organs during acute liver failure in rats
- VernacularTitle:一氧化氮合酶在急性肝功能衰竭大鼠主要器官中表达的变化
- Author:
Jianmin QIN
;
Yangd ZHANG
- Publication Type:Journal Article
- Keywords:
acute liver failure;
nitric oxide;
inducible nitric oxide synthase;
endothilial nitric oxide synthase
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the expression changes of nitric oxide synthase(NOS) in the liver,the lung,the kidney and the intestine of rat during acute liver failure(ALF).Methods:Wistar male rats(n=60) were randomly divided into 6 groups:SO group,ALF 6 h group,ALF 12 h group,L-Arg (300 mg/kg) group,L-NAME (30 mg/kg) group,L-Arg+L-NAME group, each group had 10 rats.The expression of eNOS mRNA and iNOS mRNA in the liver,lung and kidney was determined with in situ hybridization,and in the small and large intestines was determined with immunohistochemical method.Results:The expression of eNOS mRNA in the lung and iNOS mRNA in the liver,lung and kidney increased significantly 6 h after operation,but the expression of eNOS mRNA and iNOS mRNA in the liver,lung and kidney reduced 12 h after operation(P
