Comparison inhibitory effect of bleomycin and carmustine on craniopharyngiomas cultured in vitro
- VernacularTitle:博来霉素与卡氮芥体外抑制颅咽管瘤的比较
- Author:
Rongcai JIANG
;
Cheng ZHU
;
Zonghui LIU
;
Zengmin TIAN
;
Hairong ZHANG
;
Yingchun ZHU
;
Ya WANG
- Publication Type:Journal Article
- Keywords:
craniopharyngioma;
bleomycin;
carmustine;
in vitro
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the inhibitory effect of carmustine(BCNU) and bleomycin on craniopharyngiomas in vitro. Methods: Cells were successfully cultured in vitro from the fresh specimens, then the culture medium with bleomycin or BCNU at different concentration was added. The tumor inhibitory curve-line was drawn based on the cell number at different time points. After cultured for 144 h, ATP-luminescence assay was applied to test the antitumor effect. Results: The cell number decreased rapidly when the medium was added. The decreasing speed was faster in BCNU medium than that in bleomycin medium at the same concentration. The bleomycin medium showed no significant inhibitory effect except for the one at 1.00 g/L. However, regardless of the concentration, BCNU medium inhibited the cells effectively. Conclusion: BCNU has stronger inhibitory effect on craniopharyngiomas cells than bleomycin, it can be used to treat this tumor
