Improvement on micro-manipulation of fertilized eggs of mice
- VernacularTitle:小鼠受精卵显微操作方法的改进
- Author:
Jianxiu LI
;
Weijiang HU
;
Yiping HU
- Publication Type:Journal Article
- Keywords:
fertilized ovum;
survival rate;
productivity;
acquired rate;
micro-manipulation
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To improve the micro-manipulation of mouse fertilized eggs,promoting its survival rate and productivity in pseudo-pregnant mice. Methods: DNA fragments of definite concentration and size were used for micro-manipulation with conventional and improved methods, then their survival rate of manupulated eggs and productivity of pseudo-pregnant mice were analyzed and compared. Results: With improved methods, the survival rate of eggs increased from 60.3% to 79.5%, the productivity of pseudo-pregnant mice and offspring increased from 35.7% to 56.8% and 10.1% to 14.1% respectively. Conclusion: The improved methods of micro-manipulation can be used in transgenic mouse production.
