Preparation of transferrin and R8 co-modified liposome and study on its targeting to hepatoma
- VernacularTitle:转铁蛋白与R8共修饰脂质体的制备及其肝癌靶向性研究
- Author:
Lijie HAN
;
Wei LIU
;
Juan DU
;
Dongdong WEI
- Publication Type:Journal Article
- Keywords:
transferrin;
R8;
tumor targeting
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;37(4):33-35,39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare transferring and R8 co-modified liposome (TF/R8-LP)for forhepatoma targeting.Methods The co-modified liposome were prepared by film-ultrasonic method.The appearance,particle size,Zeta potential were evaluated.The cellular uptake by HepG2 cell in vitro was used to evaluate the targeting efficiency and in vivo imaging were used to evaluate the targeting efficiency. Results The particle diameter of the co-modified liposome was(108.5 ±12.6)nm and the Zeta potential was(24.15 ±4.78)mV.The liposome kept stable in 50% FBS at 24 h.The result demonstrated that the co-modified liposome uptaken by HepG2 were 2.4,2.6 times higher than that of R8-LP and TF-LP,respectively(P<0.05).The evaluation of tumor spheroid penetration and in vivo imaging results showed the co-modified liposome had the strongest fluorescence intensity. Conclusion The co-modified liposome might serve as a promising hepatoma delivery system of antitumor drugs.
