THE EFFECT OF HIGH MOBILITY GROUP-1 PROTEIN ON TUMOR NECROSIS FACTOR-? EXPRESSION IN KIDNEY OF SEPTIC RATS
- VernacularTitle:脓毒症大鼠肾组织高迁移率族蛋白-1对肿瘤坏死因子-?表达的影响
- Author:
Yongming YAO
;
Litian ZHANG
;
Jiaq LU
- Publication Type:Journal Article
- Keywords:
sepsis;
high mobility group 1 protein;
tumor necrosis factor;
sodium butyrate;
kidney failure, acute
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to investigate the effect of high mobility group 1 protein (HMG 1) on TNF ? expression as well as release in the kidney, and their potential role in the pathogenesis of acute renal injury associated with sepsis. Using a sepsis model by cecal ligation puncture (CLP), 50 male Wistar rats were randomly divided into normal control group ( n =10), CLP group ( n =20), and sodium butyrate treatment group ( n =20). At serial time points animals in each group were sacrificed, and blood as well as kidney tissue samples were harvested to determine HMG 1/TNF ? mRNA expression, tissue TNF ? protein level, and serum creatinine content. Compared to normal controls, both HMG 1 and TNF ? mRNA levels significantly increased in the kidney during 12～24h after CLP ( P
