THE ESTABLISHMENT OF AN OSTEOSARCOMA CELL LINE PL-1 AND ITS BIOLOGIC CHARACTERS
- VernacularTitle:具有肺转移特性的人骨肉瘤细胞系PL-1的建立及其生物学特性观察
- Author:
Lei PENG
;
Zhen WANG
;
Yuny HU
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
tumor cells, cultured;
biological characteristics
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
To establish a xenografted tumor strain of human osteosarcoma in nude mice and a homologous cell line, the osteosarcoma speciments from patients were inoculated into the subcutaneous tissue of nude mice. After the transplanted tumor had grown to about 2cm in diameter, the tumor tissue was cultured in vitro and the continuously growing cells were analyzed by morphology, histochemistry, immunohistochemical straining, cell cycling analysis ,chromosome analysis and heterotransplantation. A newly established cell line designated PL 1 was thus obtained in this laboratory in continuous culture for over 100 passages. Under light and electron microscopes, the cells assumed the main characters of malignancy.Morphological observation, histochemistry analysis and BMP immunohisto chemical straining showed that they had the features of osteosarcoma.The cell cycle analysis showed 53 2% of the cells were in G 1. This cell line could produce ALP and BMP.Chromosome analysis confirmed that they had retained a karyotype of human cancer cells and a hypotriploid feature with a mode number of around 64～66.The tumor formation rate after heterotransplantation was 100%. It had lung metastasis characters. Therefore, PL 1 cell line derived from the xenograft in nude mice has been established and maintained for over 9 months trough 100 passages, and this cell line can provide material and model for further investigation of human osteosarcoma.
