A comparative study on the growth of Pneumocystis carinii in 8 cell lines
- VernacularTitle:卡氏肺孢子虫在八种细胞系上的增殖情况
- Author:
Xiaoyi NI
;
Yatang CHEN
- Publication Type:Journal Article
- Keywords:
Pneumocystis carinii;
Cell culture
- From:
Chinese Journal of Infectious Diseases
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the best cell line for Pneumocystis carinii(Pc) proliferation Methods Specimens containing 5?10 5 Pc cysts were inoculated onto 8 kinds of cell lines separately. At the same time, Pc were inoculated in different amounts on HepG-2 cell line to identify the most suitable number of Pc needed for Pc proliferation in vitro within seven days. Results On HepG-2 cell line, Pc increased by 7-fold in trophozoite forms with a peak increasing rate between 4～5 days with the optimal growth at the cyst number of 5?10 5 .Conclusions HepG-2 cell line is found to be the most suitable cell line for Pc culture and the best inoculation number of Pc is 5?10 5.
