Effect of rhubarb extract on gene expression profile in diabetic nephropathy rats
- VernacularTitle:大黄对糖尿病肾病大鼠肾皮质基因表达谱的影响
- Publication Type:Journal Article
- Keywords: Rhubarb; Gene expression profile; Diabetic nephropathy; Complementary DNAmicroarray
- From: Chinese Journal of Nephrology 1994;0(04):-
- CountryChina
- Language:Chinese
- Abstract: Objective To study the effect of rhubarb extract on the gene expression profile in diabetic nephropathy rats, and analyze the mechanism of rhubarb on diabetic nephropathy in gene expression level. Methods Male rats without any treatments were used as the normal control group. Diabetic nephropathy was induced by streptozotocin in the rats as the diabetic nephropathy group. The diabetic nephropathy rats were treated with rhubarb extract as the rhubarb-treated group and with placebo as negative control group. The renal cortex was obtained from four groups. Cy5 or Cy3 fluorescent labeled DNA probes were prepared from mRNA and hybridized against the BioDoor Genechip, finally fluorescent signals were assayed and raw data was analyzed. Results There were about 8. 4 % genes altered in gene expression profile. The expression level of 12 genes were downregulated while 323 genes were upregulated significantly in the renal cortex of the diabetic animals. Compared with the negative control rats, 120 genes were downregulated and eight genes were upregulated significantly in the rhubarb-treated group, which occupied 3.3% of the total genes on the genechip. Furthermore, the expression changes of partial altered genes in the diabetic nephropathy group were reversed by rhubarb treatment. Conclusion Rhubarb is effective on the downregulation or upregulation of some differential expression genes, and can partially reverse streptozotocin-induced gene expression profile.