Verapamil blocking activition of ?1(Ⅰ) collagen gene promotor induced by TGF-?1
- VernacularTitle:维拉帕米阻断TGF-?1诱导的?1(Ⅰ)胶原基因启动子的激活
- Author:
Chunhua ZHOU
- Publication Type:Journal Article
- Keywords:
verapamil;
transforming growth factor ?1;
collagen gene promotor Ⅰ;
gene expression regulation
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of verapamil on the activition of ?1(Ⅰ) collagen gene promotor induced by TGF ?1. Methods: The recombinant pCOLH 1.5 and pCOLH2.5 of ?1(Ⅰ) collagen gene promotor were transfected to rat mesangial cell and were treated with different concentration of TGF ?1 and verapamil. The activity of CAT were measured by ELISA method. Results:TGF ?1 enhanced the activity of pCOLH1.5 and pCOLH2.5(1.498 and 1.551 fold,respectively) significantly compared to the control; Verapamil inhibited the activity of the 2 recombinants. The activity of CAT stimulated by verapamil + TGF ?1 was remarkably decreased compared with the activity of pCOLH2.5 promotor by TGF ?1. Conclusion:Verapamil can not only inhibit the activity of gene promotor derectly, but also partly block the activity of ?1(Ⅰ) collagen gene promotor induced by TGF ?1.
