Identification of chemical composition in traditional Chinese medicine by liquid chromatography-mass spectrometry
- VernacularTitle:中药化学成分的液质联用鉴定法探讨
- Author:
Hui YU
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
chemical components;
LC-MS;
identification method
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(3):172-173,177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the chemical components of traditional Chinese medicine by liquid chromatography-mass spectrometry.Methods Chemical components of 86 traditional Chinese medicines were identified by two methods,observation group with LC-MS method and control group with traditional LC methods.The identification efficacy,accuracy,and identification time between two methods were compared. Results The excellent rate in observation group was 76.74% (66/86),the excellent and good rate was 90.70%(78/86),which were significantly higher than those in control group (P<0.05 ).83 kinds of traditional Chinese medicine were successfully identified in observation group,the success rate was 96.5 1%,significantly higher than control group(P<0.05).The identification tine,retention time after correction of errors in observation group were significantly less than control group(P<0.05 ).Conclusion LC-MS has high accuracy,short identification time and wide adaptation range in identification of chemical components of traditional Chinese medicine.
