IMMUNOHISTOCHEMISTRY STUDY AND LEVELS OF ENDOTHELIN-1 IN GASTRIC MUCOSA OF COLD-RESTRAINT-STRESS RATS
- VernacularTitle:应激状态下胃粘膜组织内皮素-1水平及其免疫组化研究
- Author:
Yimin DUAN
;
Zhaoshen LI
;
Weiqi WAN
- Publication Type:Journal Article
- Keywords:
stress;
gastric mucosal lesion;
endothelin 1;
immunohistochemistry;
radioimmunoassay
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the role of ET 1 in the pathogenesis of SU, a model was established by cold restraint stress(CRS) inducing rat ulcer, immunohistochemistry(IHC)ABC method and radioimmunoassay(RIA) methods were used to determine ET 1 content and to study the expression and location of ET 1 in stomach of normal and CRS rats,and to evaluate the ulcer index(UI). IHC showed that ET 1 was stained as brownish grains,localized in the cytoplasm of vascular endothelial cells, stomach smooth muscle cells, gastric epithelial and vascular smooth muscule cells around lamina muscularis mucosae in stomach. The proportion of ET 1 positive cells was significantly higher in 3h poststress gastric mucosa than in normal gastric mucosa in rats by IHC( P
