IN VITRO-INDUCED DIFFERENTIATION OF BONE MARROW STROMAL CELLS INTO NEURAL STEM CELLS AND MATURE NEURAL CELLS
- VernacularTitle:体外诱导骨髓基质细胞向神经干细胞和成熟神经细胞分化的实验研究
- Author:
Yiwu DAI
;
Ruxiang XU
;
Xiaoda JIANG
- Publication Type:Journal Article
- Keywords:
bone marrow stromal cells (BMSCs);
cell differentiation;
stem cells;
neurons
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the feasibility of in vitro inducing differentiation of bone marrow stromal cells (BMSCs) into neural stem cells and mature neural cells, and to offer reference for the application of BMSCs in the field of neuroscience, BMSCs were acquired from the marrow of dogs. Basic fibroblast growth factor (bFGF), all trans retinoic acid (RA) and glial cell line derived neurotrophic factor (GDNF) etc were used as proliferation or differentiation factors. Immunocytochemistry was employed to identify the cells at every culture stage. When BMSCs were proliferatively cultured for 24～72h, cleavage phase and cluster like clones appeared. On the 3rd day, some of the BMSCs derived cells started to express neuron specific enolase (NSE) or glial fibrillary acidic protein (GFAP). The same stage cells could be cloned, that is one of the characters of stem cells, when they were cultured in the proliferation medium. The neural cell modality like cells appeared on the 10th day after adding inducing factors into culture medium, which were proved by testing the express of NSE and GFAP. BMSCs can differentiate into neural stem cells and mature neural cells in vitro, and can be used as "seed cells" in the field of neuroscience.
