DETECTION OF MITOCHONDRIAL MEMBRANE POTENTIAL CHANGES IN CARDIOMYOCYTES BY FLUORESCENT DYES JC-1
- VernacularTitle:利用荧光探针JC-1检测心肌细胞线粒体膜电位的改变
- Author:
Yingqi LIU
;
Tiande LI
;
Xiaowe CHU
- Publication Type:Journal Article
- Keywords:
flow cytometry;
mitochondria, heart;
membrane potentials;
rats
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
To explore the changes of mitochondrial membrane potential in rat cadiomyocyte apoptosis by fluorescent JC 1, H 2 O 2 was used to induce cadiomyocyte apoptosis, and JC 1 in combination with flow cyometry was used to detect the changes of mitochondrial membrane potential in the early stage of cadiomyocyte apoptosis. The results showed that living cardiomyocytes had a high mitochondrial membrane potential, JC 1 aggregates were formed in the inner membrane of mitochondria and emitted orange red fluorescence. H 2 O 2 caused the decrease of mitochondrial membrane potential, JC 1 aggregates were dissociated to monomer,which emitted green fluorenscence. So the red fluorescence decreased. It is suggested that JC 1 in combination with flow cyometry is an ideal method to detect the changes of mitochondrial membrane potential.
