THE INFLUENTIAL FACTORS AND COMPARABILITY AMONG LABORATORIES OF RAPD ON THE IDENTIFICATION AND GENOTYPING OF BACTERIA
- VernacularTitle:随机扩增多态性DNA在细菌鉴定分型中的影响因素
- Author:
Rui ZHAO
;
Jun WANG
;
Yueq LI
- Publication Type:Journal Article
- Keywords:
randomly amplified polymorphic DNA;
bacteria typing technique;
quality control
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the influential factors of different concentrations of some reagents and the comparability among the laboratories of RAPD. RAPD was carried out with different concentrations of reagents and three different amplified cyclers to amplify 11 strains of pathogenic vibrios. 11 strains of pathogenic vibvios were genotyped into 4 types, and could be easily repeated.The different concentrations of primer and 4 dNTP could influence the result of RAPD,but the fingerprints of RAPD produced respectively with 3 amplified cyclers were consistent. It is suggested that RAPD can be compared among the laboratories with the standard reagents.
