Bile leakage in transjugular intrahepatic portosystemic shunt: effect on growth and function of endothelium
- VernacularTitle:经颈静脉肝内门腔静脉分流术中胆汁漏出对内皮细胞生长及功能的影响
- Author:
Qin LU
;
Gaojun TENG
- Publication Type:Journal Article
- Keywords:
Bile;
Endothelium;
Cells, cultured;
In vitro
- From:
Chinese Journal of Radiology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of bile on growth and function of endothelial cells in vitro. Methods In vitro, endothelial cells explanted from human umbilical vein were cultured. The experiment was designed as 6 groups with concentration of 5%, 10%, 15%, 20%, 25% bile and without bile respectively. Morphological changes of the cells was evaluated daily until to harvest after culture. MTT value of light absorption and total protein was assessed at 5 days. von Willebrand factor (vWF) was measured for identification of cells and estimation of function. Results The explanted cells were verified as endothelial cells by vWF test. Viability and morphology of endothelial cells in culture mediums added with 5%, 10%, 15% bile were similar to those cells without bile, while cells in mediums with 20% and 25% bile were markedly reduced and immature. Comparing with the cells cultured without bile, MTT value of light absorption and total protein in mediums with 25% bile reduced statistical significantly ( P
