Preparation and pharmacokinetics study of immunoconjugate composed of Adriamycin nanoparticles and VEGFR2 monoclonal antibody
- VernacularTitle:阿霉素纳米-VEGFR2单抗交联物的制备及药代动力学研究
- Author:
Xiangbao YIN
;
Linquan WU
;
Mingwen HUANG
;
Zhiqiang LUO
;
Xin YU
;
Jun HUANG
;
Hongsong XING
- Publication Type:Journal Article
- Keywords:
VEGFR 2 monoclonal antibody;
Adriamycin;
nanoparticle
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;(2):141-143,147
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the immunoconjugate composed of Adriamycin nanoparticles and VEGFR 2 monoclonal antibody(conjugate of ADM-NP and VEGFR 2-MAb) and study its pharmacokinetics property. Methods Adriamycin nanoparticles were prepared by using double emulsion method, with PLA and O-CMC as materials. Conjugate of ADM-NP and VEGFR 2-MAb was prepared by using molecule conjugate technology. Immunoreactivity of the conjugate with type IV collagenase and H 22 cell were analyzed by using ELISA. Pharmacokinetics parameters of the immunoconjugate were obtained by using SD rats as study objects. Results The prepared ADM-NP was sphere particles under SEM, which diameters were (160±34) nm. The drug loading rate and entrapment rate were (30.15±3.5)% and (80.56±4.24)% respectively. Conjugate of ADM-NP and VEGFR 2-MAb was successfully prepared, which had immunoreactivity with type IV collagenase and H 22 cell. The immunoconjugate showed good ADM control-release ability and could prolong the retention time of ADM in vivo. Conclusion Conjugate of ADM-NP and VEGFR 2-MAb keeps the immunoreactivity of VEGFR 2-MAb and shows good ADM control-release ability.
