THE METHOD FOR CULTURE AND PURIFICATION OF NEWBORN RAT SCHWANN CELLS IN VITRO
- VernacularTitle:乳鼠雪旺细胞的培养和纯化
- Author:
Jun ZHANG
;
Bainan XU
;
Dingbiao ZHOU
- Publication Type:Journal Article
- Keywords:
Schwann cells;
purification;
cell culture
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
The aim was to look for the method for getting highly purified population and large amount of Schwann cells from the newborn rats. Sciatic nerves from 1～3 days old SD rats were taken out . The Schwann cells were cultured and purified by modified repeated explanation, and fibroblastic cells were removed by rapid trysinization , differential adhesion and anti mitosis methods. The Schwann cells were identified by indirect immunocytochemistry with mouse anti S 100 protein McAb. The result showed that the purity of Schwann cells was more than 95%, and the cells were in a good state. It is considered that the methods are simple and convenient , and a great number of highly purified Schwann cells can be acquired by them.
