Evaluation of the Automated Hematology Analyzer Sysmex XN-2000 and the Accuracy of Differential Leukocyte Counts Using the Low WBC Mode.
- Author:
Ja Young LEE
1
;
Sae Am SONG
;
Seung Hwan OH
;
Jeong Hwan SHIN
;
Hye Ran KIM
;
Kyung Ran JUN
;
Jeong Nyeo LEE
Author Information
- Publication Type:Original Article
- Keywords: Hematology analyzer; XN-2000; Differential leukocyte count; Low cell count
- MeSH: Basophils; Blood Cell Count; Blood Platelets; Cell Count; Erythrocyte Indices; Hematology*; Humans; Leukocyte Count*; Leukocytes; Lymphocytes; Mean Platelet Volume; Monocytes; Neutrophils; Platelet Count; Reticulocyte Count
- From:Laboratory Medicine Online 2015;5(4):188-195
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: The XN-series (Sysmex, Japan) is the new hematology analyzer from Sysmex, with new channels to improve the accuracy of differential leukocyte count and platelet count in the low cell count range. We evaluated the analytical performance and low white blood cell (WBC) mode of the XN-2000. METHODS: Precision, linearity, and carryover were evaluated for the analyzer. We analyzed the accordance of complete blood count (CBC), reticulocyte count, and differential leukocyte count between the XN-2000 and XE-2100 (Sysmex), using 200 samples from normal controls and patients. For 80 samples with a WBC count <1.5x10(9) cells/L, the low WBC mode was evaluated by comparing the automated count with a manual differential count as the reference. RESULTS: The coefficients of variation of precision were <5% for most CBC parameters and <10% for differential leukocyte count. All results obtained with the XN-2000 showed good correlation with those obtained with the XE-2100. The correlation coefficients (r) were >0.9800 for all CBC parameters except mean corpuscular hemoglobin concentration, mean platelet volume, and platelet distribution width, and >0.9900 for differential leukocyte count except monocytes and basophils. The low WBC mode provided accurate counts for neutrophils and lymphocytes, with r>0.9300 for samples with a WBC count of 0.1-1.5x10(9) cells/L. CONCLUSIONS: The XN-2000 showed good analytical performance and correlation with the existing model, the XE-2100. The XN-2000 provided accurate results for differential leukocyte count in samples with a WBC count of 0.1-1.5x10(9) cells/L, and reduced manual slide reviews.
