IMMUNOMODULATORY EFFECTS OF HSHRESUSCITATION ON THE DEVELOPMENT OF LUNG INFLAMMATION FOLLOWING HEMORRHAGIC SHOCK
- VernacularTitle:复方高渗盐溶液复苏失血性休克对肺部炎症反应的免疫调节作用
- Author:
Yong WANG
;
Shiming XIAO
;
Shaowe WANG
- Publication Type:Journal Article
- Keywords:
saline solution, hypertonic;
shock, hemorrhagic;
lung;
inflammation;
neutrophils;
macrophage 1 antigen (CD11b)
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
To explore the molecular mechanisms underlying the immunomodulatory effects of hypertonic sodium chloride and hetastarch 40 injection (HSH) resuscition on the development of lung inflammation in a hemorrhagic shock model, Sprague Dawley rats were subjected to hemorrhagic shock and intratracheal LPS,the so called two hit rodent model.The animals resuscitated by transfusion of either Ringer's lactate(RL) or a 6ml/kg volume of HSH were sacrificed at 1,2,4 hour after resuscitation,respectively. Surface expression of adhesion molecule CD11b on circulating polymorphonuclear (PMN) was measured by flow cytometry. Bronchoalveolar lavage fluid(BALF) neutrophil counts were determined.Lung tissue myeloperoxidase(MPO) activity reflecting total lung neutrophil infiltration was assessed by modified Bradly′ method. The results showed that resuscitation with HSH significantly reduced BALF neutrophil counts( P
