EFFECTS OF ADRENALINE AND DEXAMETHASONE ON PROLIFERATION OF THE LENS EPITHELIAL CELLS
- VernacularTitle:肾上腺素及地塞米松对白内障患者晶状体上皮细胞增殖的影响
- Author:
Tao HE
;
Yannian HUI
;
Li WANG
- Publication Type:Journal Article
- Keywords:
cataract;
lens epithelial cell;
epinephrine;
dexamethasone;
proliferating cell nuclear antigen (PCNA)
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
To detect the effects of adrenaline and dexamethasone on proliferation of the lens epithelial cells and discuss the mechanism, the central area 5～7mm in diameter of the anterior capsule was obtained in cataract surgery (phacoemulsification, Phaco), and the specimens were divided into three parts, and were cultured in vitro and treated with 10 -6 mol/L adrenaline and dexamethasone for 48 hours. And then the positive area ratio of proliferating cell nuclear antigen (PCNA) in immunohistochemistry was estimated by medical color image analysis. The data were analyzed with the statistical software. The results indicated that the 10 -6 mol/L adrenaline and dexamethasone had obvious inhibition on LEC proliferation. The positive area ratio of PCNA was (2 614?0 922)%( t =5 594, P =0 0000) and (3 338?0 838)%( t =3 361, P =0 0013). It is suggested that adrenaline and dexamethasone could inhibit the proliferation of LEC and could be used to treat PCO after cataract surgery. The experiments provide the scientific basis for selection of drugs to prevent PCO after cataract.
