Influence of vascular activator on the expression of VEGF gene in human umbilical vein endothelial cells under hypoxia.
- VernacularTitle:缺氧条件下血管活性因子对血管内皮细胞中血管内皮生长因子表达的影响
- Author:
Man ZHANG
;
Le SHEN
;
Airu ZHOU
- Publication Type:Journal Article
- Keywords:
Human umbilical vein endothelial cells Vascular activator Vascular endothelial growth factor
- From:
Chinese Journal of Practical Internal Medicine
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulation and expression of VEGF gene in human umbilical vein endothelial cells under hypoxia and the influence of vascular activators,such as endothelin(ET),nitric oxide(NO)and NO synthesis inhibitor N-nitro-L-Arginie(L-NNA),on the expression of VEGF.Methods Culture the human umbilical vein endothelial cells and then treat by hypoxia and vascular activators.The expression on VEGF mRNA level and protein level were assayed by northern blots,ELISA and computer figure analysis system respectively.Results When human umbilical vein endothelial cells were subjected to hypoxia for 6 hours,the expression of VEGF began to increase.ET could increase the expression and NO inhibited it,and LNNA affected the expression of the VEGF.VEGF protein concentration in the cell culture media matches the mRNA result.They are 6 hours hypoxia (8^2?1.1)?g/L,ET+6 hours hypoxia(9^37?1^02)?g/L,NO+6 hours hypoxia(2^86?0^91)?g/L,LNNA+6 hours hypoxia(14^75?1^87)?g/L respectively.Conclusion (1)Hypoxia can induce the expression of VEGF in human umbilical vein endothelial cells.(2)ET can increase the inducible effect of hypoxia,NO can inhibit it and LNNA stimulated the expression of VEGF in human umbilical vein endothelial cells.
