Activation-induced cell death in peripheral blood T-lymphocytes from patients with chronic hepatitis B
- VernacularTitle:慢性乙型肝炎患者T淋巴细胞激活诱导的细胞死亡
- Author:
Chunsheng HOU
;
Guiqiang WANG
;
Shulan LU
- Publication Type:Journal Article
- Keywords:
Hepatitis B, chronic;
T lymphocytes;
Apoptosis
- From:
Chinese Journal of Infectious Diseases
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the activation induced cell death(AICD) in peripheral blood T lymphocytes(PBL T) from patients with chronic hepatitis B(CHB), and the role of AICD in the pathogenesis of chronic hepatitis B. Methods The PBL Ts of 14 patients were isolated with negative selection by magnetic beads, and cultured with or without anti CD3 mAb in the presence of PMA and ionomycin. The apoptosis of PBL T was observed by TUNEL staining and assessed by Flow Cytometry. Results In CHB patients, the apoptotic rate of PBL T activated with anti CD3 mAb was significantly higher than that of without activation (16.73%?0.99% vs. 9.74%?1.14%, P 0.05), and a negative correlation existed between the level of INF? and apoptotic rate of T cell( r =-0.87126, P
