Syphilis antibody enzyme-linked immunosorbent method grey area test results analysis
10.3969/j.issn.1673-4130.2014.05.039
- VernacularTitle:酶联免疫吸附法检测梅毒抗体试验灰区结果分析
- Author:
Xucai DONG
;
Xinyang LIU
;
Jing ZHANG
;
Man WANG
;
Luan LI
;
Huizhong ZHANG
- Publication Type:Journal Article
- Keywords:
syphilis;
enzyme-linked immunosorbent assay;
syphilis serodiagnosis
- From:
International Journal of Laboratory Medicine
2014;(5):598-598,600
- CountryChina
- Language:Chinese
-
Abstract:
Objective ELISA syphilis enzyme analysis(TP-ELISA) results for the gray zone two step after the replacement .To explore the suitability of syphilis detection procedures ,reduce misdiagnosis .Methods Randomly choose 50 288 cases of serum screening for syphilis enzyme linked immunosorbent assay ;in grey area of the sample ,then rapid plasma reagin test (RPR) and Treponema pallidum particle agglutination test(TPPA) for validation .According to disease and age groups ,statistics of anti TP an-tibody gray area ratio .Results 50 288 serum samples ,after TP-ELISA method to screen grey area in the early results of 61 cases . The highest age of grey area rate in different ages was more than 61 years old .Conclusion TP-ELISA detection still exist certain false positive ,set reasonable grey area ,the gray zone within the sample confirmation test has important clinical significance .
