Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry analysis of differential serum proteins in breast cancer patients with bone metastasis
10.3969/j.issn.1671-8348.2014.05.021
- VernacularTitle:乳腺癌术后骨转移患者血清差异蛋白的质谱分析
- Author:
Peng ZHANG
;
Baoleri XILIN
;
Jingping BAI
;
Renbing JIANG
;
Yongzhong CHEN
;
Zhe HUANG
;
Xing LI
- Publication Type:Journal Article
- Keywords:
breast neoplasms;
bone metastasis;
differential protein;
Clinprot system
- From:
Chongqing Medicine
2014;(5):566-568,571
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain the peptide mass fingerprintings(PMF)of serums of the patients with bone metastasis and with-out metastasis and to filtrate and identify the differential serum proteins of patients with bone metastasis after radical mastectomy . To establish diagnostic models for diagnosis of bone metastasis after breast cancer operation .Methods Two groups of serum sam-ples were analyzed by ClinprotTM MALDI-TOF MS and gain PMF ,18 samples from patients with merely bone metastasis and 18 samples from patients without metastasis .Characteristic protein peaks were analyzed and selected by analyses software within Clin-prot system .Every group was repeated 2 times .Results All serum samples were repeated after 5 days and the fingerprintings were similar to the former .4 protein peaks were selected randomly to compute coefficient of variation which are less than 30% .The Clin-prot system has excellent repeatability .No differential protein was detected by analyzing PMF (P>0 .05) .Conclusion No differen-tial serum protein exists in patients with bone metastasis and without metastasis and detecting differential protein in peripheral blood may be impossible .
