Suppressive effect of nickel-titanium stent on macrophage cells reactivity to lipopolysaccha-ride(LPS) from gram-negative bacterium
10.3969/j.issn.1671-8348.2014.05.024
- VernacularTitle:镍钛合金支架抑制单核巨噬细胞应答革兰阴性菌脂多糖的研究
- Author:
Haibo LUO
;
Aizhen LV
;
Xiao LU
;
Ning FU
;
Sha WU
;
Hua ZHENG
- Publication Type:Journal Article
- Keywords:
dexamethasone;
lipopolysaccharides;
nickel titanium stent;
macrophage cells
- From:
Chongqing Medicine
2014;(5):575-577,581
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of nickel-titanium stent(NTS) and consequent anti-allergy dexamethasone therapy on macrophage cells reactivity to lipopolysaccharide (LPS) from gram-negative bacterium .Methods The macrophage cell line Raw 264 .7 and dexamethasone-pretreated Raw264 .7 were co-cultured with NTS for 4 days ,and stimulated with LPS for 24 hours .The surface marker CD molecules of CD80 ,CD86 and FasL were detected with flowcytometr method ,the supernant cytokine production of proinflammatory cytokines IL-6 and TNF-αwas valued with ELISA method ,and intracellular inflammatory signal pathway acti-vation of NF-κB ,GAS ,ISRE and STAT3 was checked with signal molecule specific promoter lunciferase analysis .Results The stent pre-treatment improved LPS-mediated CD80 expression ,suppressed FasL production ,decreased IL-6 secretion and NF-κB ac-tivation ,the results have statistical significance (P<0 .05) .The dexamethasone treatment improved stent-mediated up-regulated ex-pression of CD80 ,FasL and TNF-α,and suppressed the activation of intracellular inflammatory signal pathway of NF-κB ,ISRE and STAT3 ,the results have statistical significance(P<0 .05) .Conclusion NTS inhibit macrophage cells Raw264 .7 react to TLR4 ag-onist LPS ,and dexamethasone treatment improved the function .
