Screening for genes related to novel gene AngRem104 by gene chip
- VernacularTitle:采用基因表达谱芯片技术筛选新基因AngRem104的功能相关基因
- Author:
Xiubin LIANG
;
Hong ZHANG
;
Haiyan WANG
- Publication Type:Journal Article
- Keywords:
Angiotensin Ⅱ;
Fibronectin;
Gene chip;
AngRem
- From:
Chinese Journal of Nephrology
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the genes related to novel gene AngReml04. Methods Gene chip was employed to detect the genes related to AngRem104 by its over-expressed constructs, which was produced by transfection of the sense- and antisense-AngReml04 into human mesangial cells, and then RT-PCR was used to confirm the up-regulated expression of related genes. Results There were 94 genes up-regulated and 2 genes down-regulated when AngRem104 was over-expression. The different expression genes were cataloged as extracellular matrix and receptor protein, DNA-binding and transcription-related protein, immune-related protein, cytoskeletal and dynamic protein, synthesis and metabolism related protein. Fibronectin (FN) and integrin beta 1 (fibronectin receptor) were dramatically over-expressed in the top of all up-regulated genes. Furthermore, the correlative expression between AngReml04 and FN was detected by RT-PCR. Conclusion Gene chip not only demonstrates the clues for further investigation of novel gene AngRem 104, but also reveals the co-expression of AngRem104 and fibronectin.
