Expression of hepatocyte growth factor receptor in flbroblasts induced by high glucose
- VernacularTitle:肝细胞生长因子受体在高糖诱导肾间质成纤维细胞中的表达及其意义
- Author:
Shan MOU
;
Qingyi ZHANG
;
Zhaohui NI
- Publication Type:Journal Article
- Keywords:
Hepatocyte growth factor receptor;
Fibroblast;
Diabetic nephropathy;
Receptor-ligand system
- From:
Chinese Journal of Nephrology
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of hepatocyte growth factor(HGF) receptor (c-met) and investigate the changes in activity of the HGF/c-met system in human kidney fibroblast by high glucose. Methods HGF and c-met mRNA levels in fibroblast induced by high glucose were detected by RT-PCR. C-met protein was examined by Western blotting. At the same time, the expression of TGF-? and c-met in the exogenous HGF and treating with anti-c-met antibody in vitro were measured. Results Extremely rapid induction of HGF and c-met mRNA was observed at the first six hours by high glucose. On the other hand, both c-met mRNA and c-met protein were markedly increased. HGF (50 ng/ml) induced the expression of c-met ( P
