Hypoxia stimulates vascular endothelial growth factor production in human renal flbroblasts
- VernacularTitle:缺氧诱导人肾间质成纤维细胞血管内皮生长因子的表达
- Author:
Heng LI
;
Zhihong LIU
;
Dong LIU
- Publication Type:Journal Article
- Keywords:
Fibroblast;
Hypoxia;
Vascular endothelial growth factor
- From:
Chinese Journal of Nephrology
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hypoxia on vascular endothelial growth factor (VEGF) expression and secretion in human renal fibroblasts. Methods Human renal fibroblasts were primary cultured from donor kidney, and cells in passage 3 were used in this experiment. Hypoxia was induced by incubation in a gas-tight box gassed with 10% CO2/10% H2/80% N2 for 24 hours. VEGF mRNA levels were measured by semi-quantitative RT-PCR method. VEGF proteins expressed on cell surface were measured by flow cytometry. VEGF proteins secreted into supernatants were measured by ELISA method. Results Hypoxia caused a 2. 5-fold increase of VEGF mRNA level in human renal fibroblasts. Hypoxia also induced significant increases of VEGF proteins on cell surfaces (mean fluorescence intensities: 1. 053?0. 055 vs 0.763?0.057, n=4,P
