The study on the expression pattern of MMP-2 and E-Cadherin in the effects of gastrin on gastric cancer cells

VernacularTitle:胃泌素对胃癌细胞MMP-2和E-Cadherin表达模式的影响
Author: Yuqing ZHANG ; Youhui XU ; Wenting LI ; Xuesen ZOU ; Daya LUO ; Wenxue CHEN ; Xiuzhen HUANG
Publication Type:Journal Article
Keywords: gastrin; proglumide; methylation; matrix metallo proteinase 2; cadherins
From: Chongqing Medicine 2013;(36):4374-4376,4379
CountryChina
Language:Chinese
Abstract: Objective To approach on the expression pattern of MMP-2 and E-Cadherin in the effects of gastrin on gastric canc-er cells ,so as to investigate their role in the metastasis and invasion of gastric cancer .Methods Genomic DNA modified by sodium bisulfite was used as a template .RQ-MSP detect the methylation status of the gene promoter .The relative amount of Methylation status was performed with comparative threshold cycle (2- △ t ) method .Results MMP-2 and E-Cadherin genes in gastric cancer cells were at hemimethylated status in gastrin before and after the intervention .Gastrin can reduce the methylation levels of MMP-2 gene(P<0 .05) ,but the could be effected by proglumide receptor antagonist to some certain extent (P>0 .05) .Gastrin can pro-mote the methylation levels of MMP-2 gene ,but proglumide decrease that effect (P<0 .05) .Conclusion Gastrin plays a certain role in the biological behavior of gastric cancer impact by changeing on the expression pattern of MMP-2 and E-Cadherin in the effects of gastrin on gastric cancer cells .