HUMAN BONE MORPHOGENETIC PROTEIN 7 EXPRESSION IN RABBIT BONE MARROW STEM CELL TRANSFERRED BY RETROVIRAL VECTOR MEDIATED hBMP_7 GENE
- VernacularTitle:逆转录病毒介导hBMP_7基因转染兔骨髓间充质干细胞的表达
- Author:
Dan JIN
;
Weisen ZENG
;
Guoxia PEI
- Publication Type:Journal Article
- Keywords:
bone marrow stem cell;
bone morphogenetic proteins;
biomedical engineering
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The human bone morphogenetic protein 7 (hBMP 7) gene was reconstructed in retroviral vector and transferred into incasing cells PT67 by liposome mediated method.The clones of the cells transferred with BMP 7 were selected by G418, and targeted rabbit bone marrow stem cells were infected with the virus granules which secreted from PT67 cells and also selected by G418. The mRNA and protein of BMP 7 gene in transferred cells were analyzed with hybridization in situ and immunohistochemistry. BMP 7 retrovirus vetor was successfully reconstructed. Cells transferred by PLNCX 2 hBMP 7 expressed abundant human BMP 7 mRNA and protein in the cytoplasm. However positive findings were not found in those cells that were not transferred. It may be used to increase the osteogenic capability of BMSc in the study of bone tissue engineering.
