ROLE OF PS_2,TGF-?,COX-2 IN STRESS GASTRIC MUCOSAL ADAPTIVE CYTOPROTECTION
- VernacularTitle:PS_2、TGF-?和COX-2在应激胃粘膜适应性细胞保护中的作用
- Author:
Zhaoshen LI
;
Shinan NIE
;
Xianba ZHAN
- Publication Type:Journal Article
- Keywords:
stress;
gastric mucosa;
cytoprotection;
genes
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To determine the role of mucosal gene expression for PS 2 (a member of trefoil peptides), transforming growth factor alpha(TGF ?) and COX 2 during gastric adaptation to WRS in rats, Wistar rats were exposed to single or repeated WRS for 4h every other day for up to 6 days. Gastric mucosal blood flow(GMBF) was measured by LDF 3 Flowmeter,the extent of gastric mucosal lesions was evaluated grossly and histologically, and the expression of PS 2, TGF ?,COX 2 mRNA determined by RT PCR,was used to further demonstrate pS 2 and TGF ?,their expression was detected by immunohistochemistry.The results showed that WRS applied once resulted in numerous gastric mucosal erosions,but repeated application WRS ,could induce adaptation to it. The area of gastric mucosal lesions was reduced by 21 99% after four consecutive WRS. This adaptation to WRS was accompanied by increased GMBF and active cell proliferation in the neck region of gastric glands and by increased expression of PS 2 (0 77?0 01 vs 0 37?0 02, P
