CONSTRUCTION OF THE MUTANTS OF HUMAN PEPTIDE ANTIBIOTICS hPAB-? AND ITS RECOMBINANT BACULOVIRUS
- VernacularTitle:人源肽抗生素hPAB?突变体及其重组杆状病毒的构建
- Author:
Xiancai RAO
;
Zhijin CHEN
;
Xiaoli JIN
- Publication Type:Journal Article
- Keywords:
peptide;
antibiotics;
mutant;
baculovirus, recombinant
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The aim was to determine the antimicrobial activities of peptide antibiotics hPAB ? and construct the recombinant baculovirus of its mutants. Four mutants of hPAB ? were designed based on molecular autosyndetic modeling and its genes were cloned by PCR. The transfer plasmid and recombinant baculovirus were constructed. The results showed that the hPAB ? cloned previously, is a good peptide antibiotics. Four mutant genes of hPAB ? were inserted into pFAST HTa plasmid and the recombinant pFAST hPAB ? were screened by restriction enzymes analysis and DNA sequencing. The recombinant baculovirus was obtained after transforming pFAST hPAB ? into Escherichia coli DH10Bac. Our work lays a good foundation for further research.
