MODULATION OF ANTISENSE PHOSPHOROTHIOATE OLIGODEXYNUCLEOTIDES OF LAMININ RECEPTOR ON MMP-2、MMP-9 mRNA EXPRESSION IN HUMAN BILE DUCT CARCINOMA
- VernacularTitle:胆管癌细胞层粘连蛋白受体表达下调对蛋白水解酶MMP-2、MMP-9 mRNA表达的影响
- Author:
Changhong CHEN
;
Shuguang WANG
;
Dajian LI
- Publication Type:Journal Article
- Keywords:
receptors, laminin;
oligonucleotides, antisense;
cholangiocarcinoma;
matrix metalloproteinases;
neoplasm metastasis
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to investigate modulation of phosphorothioate oligodexynucleotides of laminin receptor on MMP 2 and MMP 9 gene expression in human bile duct carcinoma cells. Treated with a concentration of 12?mol/L LNR AS OD in cuture medium, the relative MMP 2 and MMP 9 mRNA level at 72h in human bile duct carcinoma cells was evaluated by reverse transcriptase polymerase chain reaction. The results showed that the expression of MMP 2 and MMP 9 dropped obviously by AS OD group as compared with control group. The relative expression level of MMP 2 and MMP 9 gene mRNA decreased about 33 2% and 23 9% respectively. It is suggested that LNR AS OD is the regulator of MMP 2 and MMP 9 gene expression. It can decrease MMP 2 and MMP 9 gene expression in human bile duct carcinoma cells. Understading the regulation of MMP 2 and MMP 9 gene expression in human bile duct carcinoma cells might contribute to the development of a new preventive and theraputic strategy for tumor invasiveness.
