CULTURE OF MENISCAL CARTILAGE CELLS AND THEIR BIOLOGICAL BEHAVIORS in vitro
- VernacularTitle:人半月板纤维软骨细胞培养及生物学特性研究
- Author:
Yunli ZHU
;
Xin JING
;
Zhih MIAO
- Publication Type:Journal Article
- Keywords:
human;
meniscus;
fibrocartilage;
cell culture
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the behavior of meniscal fibrocartilage cells in vitro ,human meniscal fibrocartilage cells were harvested from adolescents and sequentially digestied with 0 50% trypsin and 0 2% collagenase (type2). The isolated cells were cultured in Ham'sF 12 medium and identified by histochemical methods. Cell proliferation, morphology and ultrastructure were observed by phase contrast microscope and electronic microscope. In primarily cultured cells, fusiform cells dominated and they were confirmed by the positive result of immunohistochemical examination for type collagen staining. Our results indicate that it is a simple and highly effective method.
