CONSTRUCTION OF TGF-?_1 MATURE PEPTIDE GENE AND ITS EXPRESSION IN ESCHERICHIA COLI
- VernacularTitle:TGF-?_1成熟肽基因克隆及在大肠杆菌中表达
- Author:
Daming SUN
;
Shuxun HOU
;
Guixi DU
- Publication Type:Journal Article
- Keywords:
transforming growth factor beta;
gene rearrangement;
gene expression;
plasmid
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
To further understand the function and biological activity of TGF ? 1, and to provide the basis for the production of biologically active TGF ? 1 protein, TGF ? 1 cDNA was cloned into procaryotic expression vectors--pBV220 and pQE30, and the monomeric form of the recombinant TGF ? 1expressed in E.coli DH5? and M15. Sephacryl S 100 HR and Ni + NTA agarose column were used to purify the TGF ? 1 protein. With digestion of the enzymes and sequencing, TGF ? 1 mature peptide gene was inserted into procaryotic expression vectors--pBV220 and pQE30. After purified, the expressed products of two plasmids in E.coli showed a single protein on SDS--PAGE, and their expression levels were about 15% and 20% of the total bacterial protein. The construction of recombinant plasmids and preparation of the monomeric protein of TGF ? 1 lay a solid foundation for further studying the function of TGF ? 1 and producing biologically active TGF ? 1 protein.
