CLINICAL SIGNIFICANCE OF THE COMBINED DETECTION OF K-RAS GENE MUTATIONS AND CA19-9 IN PLASMA FROM PATIENTS WITH PANCREATIC ADENOCARCINOMA
- VernacularTitle:胰腺癌患者外周血浆中Kras基因突变和CA19-9联合检测的临床意义
- Author:
Wenyao WANG
;
Xiangui HU
;
Guison CAO
- Publication Type:Journal Article
- Keywords:
pancreatic neoplasm;
genes, ras;
CA19 9;
polymerase chain reaction;
tumor markers, biological
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Two stage polymerase chain reaction(restricted fragment length polymorphism was used to study K ras mutations and chemiluminescence immunoassay was used to detect CA19 9 in plasma from patients with pancreatic adenocarcinoma, some samples were analyzed by direct seqencing. The results showed 18 (60%) of 30 patients with pancreatic adenocarcinoma had K ras mutations in plasma, and 25 (83 3%) of 30 had elevated CA19 9 in plasma. The former was less sensitive than the latter, but the diagnostic rate of combined analysis was 100%. It is concluded that combined analysis of molecular tumor markers and protein tumor marker is more sensitive than protein tumor markers alone, it might overcome Lewis blood type limitation to protein markers.
