Research of screening early diagnostic biomarkers of pancreatic cancer using 2D-DIGE and MALDI-TOF-MS analysis
10.3760/cma.j.issn.1673-4203.2013.04.007
- VernacularTitle:应用双向差异凝胶电泳和质谱技术筛选胰腺癌血清早期诊断标志物
- Author:
Wen WU
;
Jiong CHEN
;
Longjiang CHEN
;
Hangcheng ZHOU
;
Renbao YANG
;
Liwei HU
;
Yue ZHAO
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Proteomics;
Diagnosis;
Tumor markers
- From:
International Journal of Surgery
2013;(4):242-246,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective A comparative proteomic method was utilized to analyze serum proteins among pancreatic cancer patients,pancreatic benign tumor group,chronic pancreatitis group and normal control group to discover a new potential specific early diagnostic marker.Methods Comparative analysis on the pancreatic peripheral blood protein profiling from 40 pancreatic cancer patients,10 benign tumor patients,10 chronic pancreatitis patients and 40 cancer-free controls from May 2009 to April 2011 was carried out by 2D differential gel electrophoresis (2D-DIGE) and differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).Results Three differentially expressed proteins,Hemopexin (Hpx),Ficolin 3 (FCN3) and Serum amyloid P-component (SAP) was identified.Those proteins were higher expression in pancreatic cancer group compared with benign tumor group,chronic pancreatitis group and normal control group.Each point in pancreatic cancer expression were 1.57,1.99,1.63 times than normal control expression,respectively (P ＜0.05).Conclusions In this study,the identified proteins,Hpx,FCN3 and SAP may be as potential specific early diagnostic markers of pancreatic carcinoma.2D-DIGE and MALDI-TOF-MS technology in screening specific serum tumor markers of pancreatic cancer has a well repeatability and stability.
