Study on the immunogenicity of Schistosoma japonicum mitochondria related protein
- VernacularTitle:日本血吸虫重组线粒体相关蛋白免疫学活性鉴定
- Author:
Xuemei HU
;
Zhaosong ZHANG
;
Chuan SU
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Mitochondria;
Recombinant fusion proteins;
Immunoprotection;
Inclusion bodies
- From:
Chinese Journal of Infectious Diseases
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the immunogenicity and the potential of using Schistosoma japonicum mitochondria related protein(rSj338) as a candidate vaccine antigen for Schistosomiasis japonica . Methods The expressed recombinant fusion protein(rSj338/26GST) was purified and recognized by S.japonicum heavily infected rabbit sera and rSj338/26GST immunized rabbit sera by Western blotting. The inclusion bodies,the revivified protein and the purified protein from S 12 gel filtration were injected twice into rabbits to produce anti rSj338/26GST antibody which titer was then measured by dot ELISA. Balb/c mice were immunized with the inclusion bodies and the purified protein from S 12 gel filtration and challenged with S. japonicum cercariae to identify the protective immunity produced by rSj338/26GST. Results The inclusion bodies, the revivified protein and the purified protein from S 12 gel filtration could stimulate the rabbits to produce high level of anti rSj338/26GST antibodies and could be recognized by S. japonicum heavily infected rabbit sera and rSj338/26GST immunized rabbit sera. In Balb/c mice, the inclusion bodies could induce 27.8%( P
