Evaluation on expression of recombinant S gene of human hepatitis B virus in vitro and in vivo
- VernacularTitle:重组人乙型肝炎病毒S基因表达的体内外评价
- Author:
Ruiyi YANG
;
Qingping ZENG
;
Linchun FU
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
S gene;
Vaccines DNA;
Gene expression
- From:
Chinese Journal of Infectious Diseases
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a DNA vaccine capable of expressing S gene of hepatitis B virus(HBV) and evaluate the expression of recombinant S gene in vitro and in vivo. Methods A cloned S X gene fragment was inserted into a eukaryote expression vector to construct a recombinant plasmid. The S gene was transcribed in vitro and expressed in a transfected cell line, and the efficiency of HBsAg in eliciting anti HBs was evaluated in mice. Results The expression of S gene was confirmed by Northern blotting, Western blotting, and ELISA(for both antigen and antibody detection). Conclusions The recombination and expression of S gene is achieved successfully in vitro.
