Effect of losartan on the level of nitric oxide in renal tissue of diabetic rats
- VernacularTitle:洛沙坦对糖尿病大鼠肾组织一氧化氮水平的影响
- Publication Type:Journal Article
- Keywords: Diabetes mellitus; Diabetic nephropathy; Losartan; Nitric oxide; Inducible nitric oxide synthase
- From: Chinese Journal of Nephrology 1994;0(02):-
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of losartan on inducible nitric oxide synthase (iNOS) and nitric oxide (NO) in diabetic renal tissue of rats. Methods Male Wistar rats were divided into 3 groups, group A( n = 11) : control; group B( n = 11) : without any therapy, and group C( n = 9) : treated with losartan. Diabetic models were made by the intraperitoneal injection of streptozotocin. At the end of the 18th week, kidneys were taken out from all the rats to measure the local level of NO and the expression of iNOS mRNA by RT-PCR with the p-actin as the internal control. Also, glomerular basement membrane (BM) thickening and mesangial matrix (MM) density (MM area/mesangial area) of kidneys were observed by electronic microscope. At the same time, blood was drawn from the heart to measure the level of creatinine, and 24-hour urine was collected to measure the level of creatinine and albumin (urine albumin excretion, UAE) by rat albumin enzyme immunoassay kit. Results The expression of renal iNOS mRNA in group B (0. 30?0. 12) was significantly higher than that in group A (0. 12?0. 04, P 0. 05) . The level of renal NO in group B [(0. 56 ?0. 20)umol/mg]was significantly lower than that in group A[ (1. 05?0. 25)umol/mg]and group C[(l.13 ?0.62)umol/mg, both P