Effect of theasinesin on proliferation and apoptosis of murine lymphocytes1
- VernacularTitle:聚酯型儿茶素对小鼠淋巴细胞增殖和凋亡的影响
- Author:
Xiaoyan SHI
;
Huawen XIN
;
Bijun XIE
;
Fandian ZENG
- Publication Type:Journal Article
- Keywords:
theasinesin;
apoptosis;
thymocyte;
splenocyte;
mouse
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of theasinesin(TS) on proliferation and apoptosis of murine lymphocytes. METHODS: MTT method was employed to investigate the proliferatory response of murine splenocytes. Cellular morphological observation, DNA agarose gel electrophoresis and flow cytometry analysis were used to study the effects of TS on apoptosis of murine thymocyte and splenocyte. RESULTS: 50,150 and 500 mg?L -1 TS could markedly promote ConA-induced splenocyte proliferation in mice. In the cellular morphologic observation and DNA biochemical analysis, murine thymocytes cultured for 20 h in vitro showed keryopyk-nosis, condensation of cell, typical "ladder" on gel electrophoresis and typical apoptotic peak (AP) by FACS analysis. After treatment with 50, 150 and 500 mg?L -1 TS , DNA ladder and apoptotic peak were decreased gradually and apoptotic rates were reduced to 12.14%, 9.49%, 6.71% respectively from 19.87% in control group. But the apoptosis of murine splenocytes induced by 10 -5 mol?L -1 DEX was not affected by TS. CONCLUSION: TS can significantly promote splenocyte proliferation in mice and has an inhibitory effect on spontaneous apoptosis of murine thymocytes.
