Comparison of CYP2D6 genotyping by allele-specific PCR with DXT phenotype and gene chip testing
- VernacularTitle:CYP2D6PCR基因型与DXT表型和基因芯片检测的比较(英文)
- Author:
Fengxiang YAN
- Publication Type:Journal Article
- Keywords:
CYP2D6;
phenotype;
genotype;
PCR;
oligonucleotide microarray hybridization
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate association of genotype and phenotype of CYP2D6 and the application of oligonucleotide microarray hybridization genetic testing in CYP2D6 multiple alleles analyses. METHODS: Two hundred forty-two healthy volunteers were recruited, and a 60 mg oral dose of dextromethorphan (DXT) was administered to each for assessment of the DXT metabolic ratio . A 20 ml blood sample was also collected for DNA isolation and testing. CYP2D6 alleles *2--*11; *17 and multiple CYP2D6 gene copies were tested by allele-specific PCR and/or the affymetrix CYP450 gene chip assay. Five of the CYP2D6 alleles (*3, *4, *6, *7, and *9) were evaluated by both PCR and the CYP450 gene chip assay. RESULTS: The CYP2D6 genotype was more informative and reflective in CYP2D6 enzyme expression than a phenotype. Genetic tests for the CYP2D6 *3, *4, *6, *7 and *9 alleles showed a high degree of concordance between the CYP450 gene chip and AS-PCR methods. CONCLUSION: Oligonucleotide microarray hybridization is a promising and reliable approach for detecting multiple alleles at gene loci.
