Effects of chloride channels blockers on Ca~(2+)influx induced by adrenoline in A10 cells
- VernacularTitle:Cl~-通道阻断剂对A10细胞?_1-AR介导的Ca~(2+)内流的影响
- Author:
Hongmei RUAN
;
Yongyuan GUAN
;
Hua HE
;
Qingying QIU
- Publication Type:Journal Article
- Keywords:
vascular;
Cl -channels;
cell;
Ca 2+
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the relationship between chloride channels and the Ca 2+influx induced by adrendine(Adr). METHODS: The effects of drugs on Adr-induced Ca 2+influx were investigated with Fura-2 fluorescence technique. RESULTS: Adr-induced Ca 2+influx was inhibited by nifedipine,SK&F96365,NFA and furosemide in a concentration manner respectively; Ca 2+influx could be further inhibited by NFA or furosemide after the maximal inhibition by SK&F96365;SK&F96365 also could further inhibit the Ca 2+influx which had been inhibited by NFA or furosemide. Genistein and vanadate could reduce or increase the Ca 2+influx respectively. CONCLUSION: Ca 2+influx induced by Adr is related to VDC and ROC, and chloride channels involves in the processes.The levels of tyrosine phosphoralation affect the Ca 2+influx.
