BONE RECONSTRUCTED BY SEEDING HUMAN BONE MARROW STROMAL CELLS ON POROUS CERAMIC
- VernacularTitle:培养人骨髓基质细胞接种多孔陶瓷再造骨组织
- Author:
Qichun QIAN
;
Yanpu LIU
;
Fuli CHEN
- Publication Type:Journal Article
- Keywords:
bone marrow stromal cells;
hydroxyapatites;
osteogenesis;
tissue engineering
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
To assess the ability of new bone formation with composite human bone marrow stromal cell (HBMSC)/coralline hydroxyapatite(CHA) in vivo. Human bone marrow collected from adult ilium by needle aspiration was cultured in vitro to expand and differentiate the stromal cells. The cells were seeded on CHA discs, then the HBMSC/CHA composites were implanted subcutaneously into athymic nude mice (CHA alone as control). At 4 and 8 weeks after implantation, the effectiveness of bone formation was evaluated by means of gross and histological observations. The results showed that HBMSC/CHA composite could form bone tissue in vivo, in which a small amount of HBMSC could be used as seed cells in bone tissue engineering, and CHA could be used as a kind of scaffold.
