THE APPLICATION OF MOLECULAR MODELING OF HLA ANTIGENS IN UNMATCHED STEM CELL TRANSPLANTATION FOR GVHD PREDICTION
- VernacularTitle:不完全相合造血干细胞移植中GVHD的预测研究:模拟HLA抗原结构的比较与应用
- Author:
Hongxing HAN
;
Fanhua KONG
;
Yongzh XI
- Publication Type:Journal Article
- Keywords:
graft versus host disease(GVHD);
molecular modeling;
stem cell transplantation;
three dimensional structure
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
To explore the pathogenic mechanism of GVHD, attempting to forecast the degree of GVHD after stem cell transplantation, and to enhance the therapeutic effectiveness of clinical transplantation. The conventional microlymphocytotoxicity and sequencing methods were used in typing the HLA. The degrees of GVHD were estimated by molecular modeling of HLA antigens and veryfied the estimation by comparing the clinical results with anticipated degrees. In 8 recipients, three were transplanted with half matched stem cells. Among these 3 pationts, two developed IV degree GVHD, and one developed II degree GVHD. In the other 3 patients, cells of two unmatched HLA antigens were transplanted, and among them one developed I degree GVHD, and two developed II degree GVHD. In two patients who were transplanted with cells of one unmatched HLA antigens, I and II degrees GVHD developed respectively. Second, the correlationship analysis showed that degrees of GVHD had positive correlation with the RMSD (relative mean square deviation) between different HLA antigens. These results indicated that the degrees of GVHD after stem cell transplantation were related with the difference of three dimensional structures of unmatched stem cell HLA antigens; molecular modeling might be used to predict the prognosis of clinical stem cell transplantation.
