CLONING AND ANALYSIS OF THE CODING SEQUENCE FOR HEPATITIS B VIRUS PRES1 BINDING PROTEIN BY T7  cDNA PHAGE DISPLAY SYSTEM

VernacularTitle:cDNA文库噬菌体展示法的建立及乙型肝炎病毒前S1蛋白结合蛋白筛检
Author: Jing DONG ; Shuangshuang SHI ; Yedong WANG
Publication Type:Journal Article
Keywords: hepatitis B virus; binding protein; phage display
From: Medical Journal of Chinese People's Liberation Army 1981;0(04):-
CountryChina
Language:Chinese
Abstract: T7 cDNA phage display method was employed to find the binding protein of PreSl protein. PreSl protein was coated in a 96-well ELISA plate, and then T7 cDNA library phages were bound to the target protein. Phages which did not bind to garget protein were washed away and the binding phages were eluted. Insertions from different clones were sequenced, and the deduced amino acid sequences were analyzed by Vector 6.0 software. Using BLAST software in GenBank, whole length of amino acid sequence of binding protein was obtained. After 4 rounds of biopanning, recombinant T7 phages with binding ablity were amplifed by infection to E. coli. One piece of amino acid sequence was found to be amino terminal of product of glioma tumor suppressor candidate region gene 2 (GLTSCR2). There was a binding domain KxPxKSGxxxL in these clones. T7 cDNA phage display technique can be used bo find the ligand. GLTSCR2 coding protein may be the binding protein to preSl protein of HBV.\;