THE COMBINING BIOLOGICAL EFFECTS OF RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2 AND DEXAMETHASONE ON BONE MARROW STROMAL CELLS
- VernacularTitle:骨形成蛋白2和地塞米松对骨髓基质细胞的联合生物学效应
- Author:
Xiaodong ZHANG
;
Xiaoming GU
;
Weidon YANG
- Publication Type:Journal Article
- Keywords:
bone marrow stromal cells;
recombinant human bone morphogenetic protein 2;
dexamethasone;
proliferation;
differentiation
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The present study was conducted to investigate the combined effects of recombinant human bone morphogenetic protein 2 (rhBMP 2) and dexamethasone (Dex) on the proliferation and differentiation of bone marrow stromal cells (BMSC). BMSCs were subjected to the treatment with different concentrations of rhBMP 2, Dex, or a mixture of rhBMP 2 and Dex, then proliferation and differentiation of the attached cells were measured with methylthiazol tetrazolium (MTT) method, Coomassie brilliant blue assay and alkaline phosphatase (ALP) measurement kit. The results showed that with treatment of rhBMP 2 alone could promote the proliferation of BMSCs and upregulate the expression of ALP in BMSCs. Treatment with Dex alone could also augment the expression of ALP, whereas high concentration of Dex could inhibit the proliferation of BMSC. Moreover, significantly greater differentiation of BMSCs were found in combined treatment group compared with individual treatment groups. The above results suggested that rhBMP 2 and Dex could synergistically promote the proliferation of BMSC and its differentiation to osteoblast.
