Absorbable gelatin sponge load neural stem cells of rats and mixed cultivating in vitro
10.3760/cma.j.issn.1001-2036.2012.04.007
- VernacularTitle:可吸收明胶海绵负载大鼠神经干细胞的体外混合培养
- Author:
Zhipeng ZHOU
;
Leping LIU
;
Qichuan ZHUGE
- Publication Type:Journal Article
- Keywords:
Neural stem cells;
Absorbable gelatin sponge;
Tissue engineering
- From:
Chinese Journal of Microsurgery
2012;35(4):285-288,后插5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the possibility of gelatin sponge as supporter of central nervous tissue engineering. Methods Primary NSCs were isolated from forebrain of neonatal Sprague Dawley rats and cuhured in serum-free medium for long-term survival in vitro. Neural stem cells were divided into absorbable gelatin sponge group and control group.Observe their morphology and proliferation.Immunofluorescence technique were used to test the results of differentiation of two groups of neural stem cells. Resultes NSCs in absorbable gelatin sponge group and control group survived and there was no conspicuous change in shape and quality. The rates of survival cell were 91.6％ and 92.8％ respectively, which was no significant difference between them.NSCs could adherented to the surface of the gelatin sponge and well-grown.After induced differentiation,NSCs started to shrink and stretch,axons grown and connected with each other,till form network structure.The expression of neuroglia cell marker GFAP and neuron cell marker NSE could be detected by immunofluorescence assay. Couclusion Neural stem cells and gelatin sponge can mixed and cultivating together in vitro.NSCs is no conspicuous change.It is suggested that absorbable gelatin sponge can serve as the carrier of the tissue engineering of central nervous system.
