ANTI-APOPTOSIS EFFECT OF NADH ON NORMAL LIVER CELL LINES TREATED WITH CYANIDE
- VernacularTitle:NADH抗肝细胞氰化物损伤的实验研究
- Author:
Xiaoping XU
;
Jiren ZHANG
;
Peng LI
- Publication Type:Journal Article
- Keywords:
nicotinamide adenine dinucleotide reduced;
cyanides;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
This study was to observe the anti apoptosis effect of NADH on a normal liver cell line inflicted with chemical hypoxia. L02 liver cells were treated with 3mmol/L KCN in the presence of different concentrations of NADH (0～600?g/ml) for 0.5, 2, 4 hours. The percentage of apoptotic cells was assayed with flow cytometry(FCM). Another three groups of experiment were done as following. Group Ⅰ was control. Groups Ⅱ and Ⅲ were treated with 3mmol/L KCN in the presence or absence of NADH (400?g/ml) for 2h. The expression of proteins of Bcl XL, Bcl 2 and Bax in the L02 cells were determined with FCM analysis. The results showed that NADH could obviously prevent L02 cells from apoptotic damage at the concentration of 400?g/ml. it could also significantly upregulate the expression of Bcl 2 and Bcl XL proteins and downregulate the expression of Bax proteins after cyanide challenge( P
